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Image Search Results
Journal: PLOS ONE
Article Title: Hydrogen decreases susceptibility to AngII-induced atrial fibrillation and atrial fibrosis via the NOX4/ROS/NLRP3 and TGF-β1/Smad2/3 signaling pathways
doi: 10.1371/journal.pone.0310852
Figure Lengend Snippet: (A) Western blot bands showing p-CaMKII together with p-RyR2. (B) and (C) The relative p-CaMKII and p-RyR2 protein expression. (D) Western blot bands of Kv1.5, Kir3.1 and Kir3.4. (E-G) The relative levels of Kv1.5, Kir3.1 and Kir3.4 proteins; (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 versus Con group; #P < 0.05, ##P < 0.01, ###P < 0.001, versus AngII group.
Article Snippet: Thereafter, primary antibodies, which included NOX4 (14347-1-AP, Proteintech, 1:1000), p22phox (sc-271968, Santa Cruz Biotechnology, 1:200), NLRP3 (ab214185, Abcam, 1:1000), ASC (sc-514414, Santa Cruz Biotechnology, 1:200), caspase1-p20 (22915–1-AP, Santa Cruz Biotechnology, 1:1,000), phospho-CaMKII (ab182674, Abcam, 1:1000), phospho-RyR2 (Ser2814,GTX00626, Genetex, 1:1000),
Techniques: Western Blot, Expressing
Journal: PLOS ONE
Article Title: Hydrogen decreases susceptibility to AngII-induced atrial fibrillation and atrial fibrosis via the NOX4/ROS/NLRP3 and TGF-β1/Smad2/3 signaling pathways
doi: 10.1371/journal.pone.0310852
Figure Lengend Snippet: (A) ROS production in HL-1 cells by immunofluorescence staining (×200). (B) Expression of NLRP3 within HL-1 cells by immunofluorescence staining (×200). (C) Intracellular Ca 2+ concentration in HL cells by fluorescent probes (×200). (D), (E) and (F) mRNAs of Kv1.5, Kir3.1 and Kir3.4 by qRT-PCR. (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001, versus Con group; # P < 0.05, ## P < 0.01, ### P < 0.001, versus AngII group.
Article Snippet: Thereafter, primary antibodies, which included NOX4 (14347-1-AP, Proteintech, 1:1000), p22phox (sc-271968, Santa Cruz Biotechnology, 1:200), NLRP3 (ab214185, Abcam, 1:1000), ASC (sc-514414, Santa Cruz Biotechnology, 1:200), caspase1-p20 (22915–1-AP, Santa Cruz Biotechnology, 1:1,000), phospho-CaMKII (ab182674, Abcam, 1:1000), phospho-RyR2 (Ser2814,GTX00626, Genetex, 1:1000),
Techniques: Immunofluorescence, Staining, Expressing, Concentration Assay, Quantitative RT-PCR